This study evaluated the production of endoxylanases by Streptomyces malaysiensis AMT-3 in submerged fermentation using by-products of the food industry at 28oC. In shake-flasks experiments, the highest endoxylanase activity of 45.8 U.mL-1 was observed within 6 days in a medium containing (w/v) 2.5% wheat bran and 1.2% corn steep liquor. The same culture conditions were used to evaluate the enzyme production in a 2 L stirred tank reactor under different agitation (300, 450 and 600 rev.min-1) and aeration (30 and 60 L.h-1) conditions. The use of 450 rev.min-1 coupled to an aeration of 90 L.h-1 resulted on 81.3 U.mL-1 endoxylanase activity within 5 days. The effect of temperature and pH on endoxylanase activity and stability showed the highest activity at 60 oC and pH 6.0. Zymography showed the presence of three xylanolytic bands with molecular masses of 690, 180 and 142 kDa. The results showed that the thermotolerant actinobacterial endoxylanase can be produced in high titers using by-product of the food industry.
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机译：该研究评估了在28oC的食品工业的副产品中的浸没式发酵中的链霉菌胺氨基脲-3的生产。在摇瓶试验中，在含有（W / V）2.5％小麦麸和1.2％玉米陡液中的培养基中在6天内观察到45.8u.ML-1的最高内毒酶活性。相同的培养条件用于评估在不同搅拌（300,450和600 in in-1）和通气（30和60μl-1）条件下的2L搅拌釜反应器中的酶产生。使用450 Rev.min-1耦合到曝气量为90L.H-1导致81.3μm-1内羰基酶活性在5天内。温度和pH对内氧羰基酶活性和稳定性的影响显示了60℃和pH6.0的最高活性。酶谱表明存在三个木聚糖带，其中分子量为690,180和142kDa。结果表明，使用食品工业的副产物，可以在高滴度中生产热电子肌肌细菌内氧基酶。
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