The effect of ginsenosides on the growth and apoptosis of human lens epithelial (HLE) B3 cells exposed to H2O2 was investigated. In addition, the effect of ginsenosides on gene expression in HLE-B3 cells was analyzed using microarray assays to determine its molecular mechanism. HLE-B3 cells were treated with 1.75?M H2O2 in the presence or absence of 5, 10 or 20?μM ginsenosides. Cell viability and apoptosis were examined by MTT assays and flow cytometry, respectively, at 24 to 120?h after the treatment. Furthermore, HLE-B3 cells were treated with 20?μM ginsenosides for 8?days and total RNA was isolated and analyzed using the Affymetrix GeneChip Array. Principal component analysis was performed to visualize the microarray data. Addition of ginsenosides significantly alleviated the growth inhibitory effect of H2O2 on HLE-B3 cells and the percentage of viable cells was increased by more than 3 folds. Flow cytometric analysis showed that 6.16?±?0.29% of H2O2-treated HLE-B3 cells were early apoptotic cells, and the percentage was reduced to 4.78?±?0.16% (P??0.05) in the presence of 20?μM ginsenosides. Principal component analysis revealed that ginsenoside caused extensive changes in gene expression in HLE-B3 cells. A total of 6219 genes showed significant differential expression in HLE-B3 cells treated with ginsenoside; among them, 2552 (41.0%) genes were significantly upregulated, whereas 3667 (59.0%) genes were significantly downregulated. FOXN2, APP and RAD23B were the top three upregulated genes while WSB1, PSME4 and DCAF7 were the top three downregulated genes in HLE-B3 cells treated with ginsenosides. Ginsenosides induce extensive changes in the expression of genes involved in multiple signaling pathways, including apoptotic signaling pathway and DNA damage response signaling pathway. Ginsenosides alleviate H2O2-induced suppression of the growth of HLB cells and inhibit H2O2-induced apoptosis of HLB cells.
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机译：研究了人参皂苷对暴露于H2O2暴露于H2O2的人晶状体上皮（HLE）B3细胞的生长和凋亡的影响。此外，使用微阵列测定分析了人参皂苷对HLE-B3细胞中基因表达的影响，以确定其分子机制。在存在或不存在5,10或20μlginsenosides的情况下，用1.75μmH2O2处理HLE-B3细胞。通过MTT测定和流式细胞术检查细胞活力和细胞凋亡，处理后24至120℃。此外，用20μmginsenoSides处理HLE-B3细胞8℃，并使用Affymetrix GeneChip阵列分离并分析总RNA。执行主成分分析以可视化微阵列数据。添加人参皂苷显着缓解了H2O2对HLE-B3细胞对HLE-B3细胞的生长抑制作用，并且活细胞的百分比增加了3倍以上。流式细胞术分析显示6.16？±0.29％的H 2 O 2处理的HLE-B3细胞是早期凋亡细胞，并且在20μm存在下，百分比减少到4.78？±0.16％（p？<β05）人参皂苷。主成分分析显示，人参皂苷引起了HLE-B3细胞中基因表达的广泛变化。总共6219个基因在用人参皂苷处理的HLE-B3细胞中显示出显着的差异表达;其中，显着上调了2552（41.0％）基因，而3667（59.0％）基因显着下调。 FOXN2，APP和RAD23B是前三个上调基因，而WSB1，PSME4和DCAF7是用人参皂苷处理的HLE-B3细胞中的前三个下调基因。人参皂甙诱导涉及多个信号通路中涉及的基因的表达的广泛变化，包括凋亡信号通路和DNA损伤响应信号通路。人参皂甙缓解H2O2诱导的HLB细胞生长并抑制H1B细胞的H2O2诱导的凋亡。
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