The function of fascinated (fas) gene was related to control fruit weight in tomato, and mutated in fas locus resulting in open reading frame alteration and created novel transcript which led to dramatically increased fruit weight. To establish a fast and reliable test method for fruit weight, identified fas gene whether wild or mutant is an available choice. This study showed that two novel transcripts were created as the result of inversion event occurred at fas locus. Based on fas gene and novel transcripts, two pairs of primers were specially designed to detect fas and novel transcripts, respectively. According to the test of genome and expression of fas, it provided an efficient method to identify fas type to screen the different fruit weight tomato without waiting long time for tomato fruit formation.
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