A novel poly (vinyl alcohol)/poly (ethylene glycol) scaffold for tissue engineering with a unique bimodal open-celled structure fabricated using supercritical fluid foaming

摘要

In this study, a novel poly (vinyl alcohol) (PVA)/poly (ethylene glycol) (PEG) scaffold was carefully designed via thermal processing and subsequent supercritical fluid (SCF) foaming. Interestingly, a bimodal open-celled structure with interconnected networks was successfully created in the plasticized PVA (WPVA)/PEG scaffold. Large cells were produced from the nucleation sites generated in the PVA phase during rapid depressurization, while plenty?of small pores generate in the cell walls of the big cells. The formation mechanism of this cellular structure was studied by considering the various phase morphologies and the diffusion behaviour of the carbon dioxide (COsub2/sub) in individual phases. In addition, the intermolecular interactions of the WPVA/PEG blend were studied using X-ray diffraction and FTIR analysis. The results demonstrate that various types of hydrogen bonds among the hydroxyl groups on the PVA chains, PEG and water molecules are formed in the blend system. The realization of thermoplastic foaming of the PVA/PEG blend benefits from the interactions of complexation and plasticization between water and PEG molecules. The SEM images also revealed that L929 fibroblast cells were able to attach and spread on surfaces of the WPVA/PEG samples. Thus the WPVA/PEG scaffold with unique bimodal cellular structure is nontoxic and favours the attachment and proliferation of cells, making it promising for use as the candidate for tissue engineering applications.