Remineralizing Efficacy of Biomimetic Self-Assembling Peptide on Artificially Induced Enamel Lesions (In vitro Study)

摘要

Background: The technology of self-assembling peptides have been progressed as an alternative remineralizing agent to fluoride, that assemble into a fibrillar three-dimensional scaffold. Aim: To investigate the effectiveness of self-assembling peptide (P11-4) on enamel remineralization both alone and in combination with flouride compared to fluoride-based delivery systems and casein phosphopeptide-amorphous calcium phosphate fluoride (CPP-ACPF). Materials and Methods: Enamel caries-like lesions were created artificially on the buccal surfaces of 25 extracted human maxillary first premolars of permanent teeth. Specimens were randomly arranged into five groups (n = 5) based on the remineralizing agent used: Group 1–control: Artificial saliva, Group 2 – fluoride varnish, Group 3 – CPP-ACPF varnish, Group 4–self-assembling peptide agent, Group 5 - double application group (self-assembling peptide and fluoride). The application of all the materials was according to their producer's instructions and the storage of the specimens were in artificial saliva that renewed daily. Assessment of surface microhardness (SMH) was done at baseline, after demineralization and after 2weeks of remineralizing agents’ application, then the data were analyzed using ANOVA and paired t-test. Results: Although, no significant difference in decrement in enamel microhardness between flouride, self-assemblig peptide and the double application group, while it was significantly higher in these groups when compared to CPP-ACPF. In addition, the control group revealed, no statistically significant difference in mean of enamel microhardness after remineralization compared to that after demineralization. Conclusions: Self-assembling peptide alone and its combination with fluoride varnish, both have the same remineralizing efficacy, showing an encouraging, noninvasive regeneration potential as an alternative remineralizing agent to fluoride.