Ganjiang granule regulates cecal microflora and serum biochemical components in a rat model of constipation-predominant irritable bowel syndrome

摘要

ObjectiveTo investigate the effects of Ganjiang granule (GG) on cecal microflora and serum biochemical components in rats with constipation-predominant irritable bowel syndrome (IBS–C).MethodsTwenty-four Sprague–Dawley rats were randomly divided into four groups: control, model, GG, and probiotics. Rats in the model, GG, and probiotics groups received 3?°C tap water intragastrically; rats in the GG group were treated with GG; rats in the probiotics group were treated with probiotics. For all rats, enzyme-linked immunosorbent assay and colorimetry were used to assess serum biochemical components related to gastrointestinal function; 16S rRNA high-throughput sequencing was used to analyze the cecal microflora.ResultsThe serum level of 5-hydroxytryptamine (HT) was higher in the model group than in the control group (Z?=??2.082, P?=?.037). The model group exhibited changes in cecal microflora: the relative abundances of Lactobacillus decreased (Z?=??2.882, P?=?.004) and Dorea increased (t?=??3.030, P?=?.023), compared with the control group. The GG and probiotics groups exhibited normal serum levels of 5-HT. The GG and probiotics groups exhibited improved serum levels of gastrin; the probiotics group exhibited an improved serum level of vasoactive intestinal peptide. Compared with the model group, The GG group exhibited greater relative abundance of Ruminococcus (Z?=??2.402, P?=?.016); the probiotics group exhibited greater relative abundance of SMB53 (Z?=??2.823, P?=?.005) and lower relative abundances of Desulfovibrio (Z?=??2.823, P?=?.005) and Facklamia (Z?=??2.608, P?=?.009).ConclusionThe effects of GG on IBS-C may be related to regulation of the serum level of 5-HT, as well as elevated relative abundance of beneficial bacteria in cecal microflora.