Change during an 8-Year Period in Streptococcus Pyogenes emm Types in Pharyngeal Isolates from Children with Noninvasive Infections

摘要

Background: Streptococcus pyogenes , or group A streptococcus (GAS), is one of the most common bacterial pathogens in children. GAS can cause such nonserious and noninvasive diseases as pharyngitis and skin infection, as well as serious, invasive diseases like streptococcal toxic shock syndrome. One factor that makes GAS pathogenic is the type-specific M protein on its cell surface. To identify emm types and their characteristics, we previously examined GAS strains isolated from children with noninvasive infections at our hospital. The present study was conducted 8 years later, for comparison. Methods: The 23 participants were inpatients and outpatients at Nippon Medical School Tama Nagayama Hospital during 2016 and 2017. A pharyngeal swab specimen was obtained from each child, and genes encoding M proteins were amplified by polymerase chain reaction. Results: emm type analysis identified emm 1 in 11 of the 23 strains and emm 12 in 4. Three group G streptococcus (GGS) strains carried M-like protein genes. Conclusions: The predominant emm type was emm 12 in our previous report and emm 1 in this study. This study also identified 3 GGS strains among the isolates, which carried either the stg 245, stg 6795, or stg 840 M-like protein gene. One GAS strain carried stg 485, a gene associated with GGS rather than GAS.