Extended-spectrum beta-lactamases (ESBLs) detection in some uropathogenic bacteria and their correlation with biofilm formation

摘要

Background and objective: The tolerance of biofilms to antibiotics results in thedissemination of resistance. Many of the recurrent urinary tract infections are assumed tobe caused by biofilm producing uropathogenic isolates. This study aimed to investigate thecorrelation between the ability of biofilm formation and extended spectrum beta lactamaseproducing uropathogens.Methods: A total of 438 urine specimens were collected from Rizgary Teaching hospital inErbil, Iraq, from September to December 2017. Extended spectrum beta lactamase wasdetermined using the Vitek-2 automated system and confirmed by using the combinationdisk diffusion test. Biofilm formation was checked using 96-well flat bottom microtiterplates.Results: Out of the 438 urine specimens, only 37.89% (n =166) developed an infection,the most common isolate was Escherichia coli. The distribution of the bacterial speciesaccording to the patients' gender found to be significant (P = 0.014). The proportion ofbetalactamase producing isolates was 29% (n = 31). The strength of biofilm formationamong Klebsiella species was significantly higher than in Escherichia coli (P 0.001), whilea non-significant difference (P = 0.163) was observed between beta lactamase productionand ability of biofilm formation. The sensitivity and specificity of VITEK-2 in the detection ofextended-spectrum beta-lactamase were 79.48% and 80.95%, respectively.Conclusion: The study revealed that Klebsiella species were stronger biofilm producers.Beta lactamase producing isolates do not have a greater ability of biofilm formation.