Bio-Efficacy of Heterorhabditis Bacteriophora (Rhabditida: Heterorhabditidae) against Serpentine Leafminer, Liriomyza trifolii Burgess (Diptera: Agromyzidae) in Oilseed Crops

摘要

Virulence and efficacy of Entomopathogenic Nematode (EPN), Heterorhabditis bacteriophora was tested against late instar larva of serpentine leafminer , Liriomyza trifoli i, under laboratory conditions. Five different Infective Juvenile (IJs) concentrations (10, 30, 50, 70 and 100 IJ larvasup-1/sup) were used. The highest mortality rate caused by H. bacteriophora is 65.1% at 24 hours and 73.8% at 48 hours post inoculation at an inoculation concentration of 100 IJ larvasup-1/sup. The minimum concentration of 30 IJs per larva shown 37.1% and 52.2% mean larval mortality at 24 and 48 hours post inoculation, respectively. The virulence of H. bacteriophora (LCsub50/sub) was established at 54 IJs at 24 hours and 37.8 IJs at 48 hours for killing 50% of the larvae tested. The results of this study revealed the potential and scope of EPNs for their utilization in management of L. trifolii in oilseed crops.