Healing effect of Shaoshang Yuhe yihao on burns in rats

摘要

Purpose: To investigate the healing effect of Shaoshang Yuhe yihao (SYY) in a deep second-degree burns model in rats. Methods: Female Wistar rats, weighing 200 - 220 g, were subjected to deep second-degree skin burns by electrical scald instrument. The animals were divided into three groups as follows: (1) second-degree burns model (control) group, (2) moist exposed burns ointment-treated group (positive control drug), and (3) SYY groups. The rats were treated with SYY for 21 consecutive days. The rate of healing, scabs dropped time and re-epithelialization time were observed every three days for 21 days after burns injury. Samples were collected from treated rats after sacrificing the animals on the 1st, 2nd, 5th, 9th, 14th, and 21st day post-burn creation, respectively. Plasma interleukin-1 (IL-1) levels were evaluated, and the tissue ex pressions of transforming growth factor-beta (TGF-β) were determined along with skin histopathology. The burned tissues were examined histologically to confirm the degree of burns and healing condition. Results: The results showed that the scabs drop time decreased (p 0.05), and healing accelerated after treatment with SYY in the burns injury rats (94.6 ± 2.6 %, p 0.05). Furthermore, the ex pressions of TGF-β significantly increased in the tissue (329.4 ± 8.2 pg/mL, p 0.05) at day 14; However, the levels of inflammatory factors in plasma decreased (11.3 ± 1.7 pg/mL, p 90 %) covered with healthy epithelial tissue and new epidermis, and showed complete epithelialization of the burns injury area. Conclusion: The results confirm the curative effects of SYY on burns injuries in rats. Thus, the herbal preparation has potentials to be developed for clinical application in burns cases.