An efficient micropropagation protocol via indirect organogenesis from callus of economically valuable crop date palm (Phoenix dactylifera L.) cultivars “Sagai and Khalas”

摘要

Regeneration of date palm cultivars (Khalas and Sagai) was achieved from callus by indirect organogenesis using different plant growth regulators (PGRs) on the modified MS culture medium. The best PGRs combination for the indirect induction of adventitious bud formation was observed at 4 mg BA/L + 0.5 mg/L of NAA in Khalas (4.50 buds) and 2 mg BA/L + 1 mg/L NAA in Sagai (8.75 buds). The multiplication of adventitious bud was found to be greater at a combination of PGRs (1 mg/L of 2iP + 0.5 mg/L of NAA) in Khalas (8 buds); and 4 mg 2iP/L + 0.5 mg NAA/L in Sagai (12.75 buds). The shootlet length (6.16 cm) was achieved at 5 mg/L GA 3 as compared to that at other GA 3 concentrations used in both cultivars. The highest number of roots (4.14 roots) from shootlets was achieved at 0.5 mg/L of NAA in cv. Sagai, whereas, in Khalas, the highest number of roots (3.28 roots) was formed at 1.5 mg/L of IBA. The highest root length (4.10 and 3.57 cm) was formed at 1.5 mg/L of IAA in both cultivars, respectively. Thus, the protocol developed through this investigation could be used at a wide scale for the production of high-quality plantlets to meet the demand of these date palm cultivars.