ASSESSMENT OF GENETIC DIVERSITY AND VARIATION OF ACER MONO MAX SEEDLINGS AFTER SPACEFLIGHT

摘要

Genetic diversity and variation of Acer Mono Maxim seedlings sampled from space-mutated (sm) populations werecompared to seedlings from parallel control (ck) ones using molecular markers. RAMP analysis showed that thepercentage of polymorphic band, Shannon diversity index and Nei’s gene diversity index of the space-mutatedpopulations were higher than those of the control ones, which indicated that genetic variation increased after spaceflightin populations of Acer Mono Maxim. By using un-weighted pair group method with arithmetic mean (UPGMA) method,three space-mutated repeats (populations) were clustered together, and control groups clustered separately, which furtherindicated that there was difference between the space-mutated ones and the control ones, which may be caused by spacemutation. Further analysis of genomic inconsistency between the root and leaf samples from the same tree showed that atotal variation rate of 6.3% and 1.7% were obtained in ten space-mutated individuals by using RAMP and SSR markers,respectively, however, the variation rate was zero in control ones. It provided that space mutation may be caused theindividual variation of Acer Mono Maxim.