EXPRESSION ANALYSIS OF FIBER RELATED GENES IN COTTON (GOSSYPIUM HIRSUTUM L.) THROUGH REAL TIME PCR

摘要

Cotton fibers are unicellular seed trichomes and the largest known plant cells. Fiber morphogenesis in cotton is acomplex process involving a large number of genes expressed throughout fiber development process. The expressionprofiling of five gene families in various cotton tissues was carried out through real time PCR. Expression analysis revealedthat transcripts of expansin, tubulin and E6 were elevated from 5 to 20 days post anthesis (DPA) fibers. Three Lipid transferproteins (LTPs) including LTP1, LTP3, LTP7 exhibited highest expression in 10 - 20 DPA fibers. Transcripts of LTP3 weredetected in fibers and non fiber tissues that of LTP7 were almost negligible in non fiber tissues. Sucrose phosphate synthasegene showed highest expression in 10 DPA fibers while sucrose synthse (susy) expressed at higher rate in 5-20 DPA fibersas well as roots. The results reveal that most of fiber related genes showed high expression in 5-20 DPA fibers.Comprehensive expression study may help to determine tissue and stage specificity of genes under study. The study mayalso help to explore complex process of fiber development and understand the role of these genes in fiber developmentprocess. Highly expressed genes in fibers may be transformed in cotton for improvement of fiber quality traits. Genes thatwere expressed specifically in fibers or other tissues could be used for isolation of upstream regulatory sequences.