EFFECTS OF AMBIENT OZONE ON REACTIVE OXYGEN SPECIES AND ANTIOXIDANT METABOLITES IN LEAVES OF PEA (PISUM SATIVUM L.) PLANTS

摘要

The differential response of two pea plants (Pisum sativum L. cultivars Little Marvel and Victory) to ambient O3 grownunder open top chambers (OTCs) was analyzed and compared. Reactive oxygen species (ROS) generation, antioxidantmetabolites such as ascorbate/glutathione as well as a series of enzymes for scavenging ROS were analyzed, all aiming toreveal the differential behavior of two closely related plants when exposed to ambient O3.Antioxidant levels and activities of related enzymes in response to ambient were noticeably different among LittleMarvel and Victory plants. However, the response was cultivar-specific. There was higher accumulation of ROS andrelatively lower induction of antioxidants and more inhibition in photosynthetic rates in Victory than Little Marvel. Therewas a good correlation between tolerance to O3 and high endogenous levels of antioxidant metabolites such as ascorbate(As), glutathione reductase (GR), superoxide dismutase (SOD), reduced (GSH) and oxidized glutathione (GSSG) in peaplants. These portrays a higher sensitivity of Victory to ambient O3.To the best of our knowledge, this is one of the very few studies attempted to describe the changes in contents ofantioxidants and activities of related enzymes in leaves of two closely related cultivars to further ourunderstanding on thedefense mechanism and strategies under ambient O3. The results highlighted the possible roles of antioxidants in O3detoxification through activation an adaptive survival mechanism allowing the plant to complete its life cycle even underoxidative stressful conditions.