Lethal effects of Ni siderophore complex on enzymatic functions in Vigna radiata under biotic and abiotic stresses

摘要

Bioremediation was employed as an innovative emerging area in the field of biotechnology; detoxify metal via complex formation with the pigments. The impact of metal, siderophore and complex were monitored separately and simultaneously both in aqueous and nutrient medium. The strains of Pseudomonas aeruginosa was selected, on the basis of their, siderophore secretion for the binding the Ni (50 & 100 ppm) from solution culture while Ni, chosen as a micronutrient, necessary for plant survival and growth, however toxic when in excesses. Bacterial responses were observed on seeds of Vigna radiata in Petri dish experiment in relation to plant growth and enzymes activity. In this regard, the enzymatic activity of roots of 4 d old seedling of Vigna radiata greatly influenced by the probable complex of Ni and siderophore of bacterial strain P. aeruginosa. Results demonstrated that altered activities of enzymes due to the presence of nickel and P. aeruginosa created an effect on growth parameters in early stages. The biomass of plant was found to be decreased in the presence of Ni and microbes when compared to uninoculated plants. The growth rate of seedling was more significant at a higher concentration of Ni which reduces in the presence of microbes that showed the apparent interaction of siderophore to that of Ni, due to which Ni was no more available for growth stimulation. The article highlights the other (biotic and abiotic) factors except for temperature and pH which influence the enzymatic functions of seedlings.