In-vitro antifungal, antibacterial, phytotoxic, brine shrimp, insecticidal activities and composition of essential oil of Tagetes minuta from Dir-Kohistan, Pakistan

摘要

The essential oil of the flowering shoot of Tagetes minuta L., collected from Dir Kohistan of Pakistan, was investigated for biological activities and chemical composition. The GC and GC/MS analysis data showed that among twenty two compounds, verbenone (25%), Unknown(11.6%),4-(5-methyl-2-furanyl)-2-butanone (8.8%) and D-limonene (8.4%) were the major constituents. The biological activity results showed that out of six bacteria stains, the oil possessed inhibitory activities against S. flexenari and S. aureus with a zone of inhibition 22mm and 20mm respectively. Among five fungal strains, only Aspergillus flavus and Candida albicans were susceptible to oil, with 60% and 30% inhibition respectively. In insecticidal tests, the oil was noted for its effectiveness toward Sitophilus oryzae , with 50% mortality. Although the oil showed no cytotoxicity against Artemia Salina , while moderate phytotoxic activity was observed for Lemna minor.