PHYSIOLOGICAL RESPONSES OF PISUM SATIVUM PLANT TO EXOGENOUS ABA APPLICATION UNDER DROUGHT CONDITIONS

摘要

This study was carried out to investigate the possibility of using foliar ABA application with different concentrations(50,100 and 150 μg/L-1) in order to decrease the adverse effect of water-deficit stress. The main parameters of interest were;growth parameters; endogenous phytohormones, proline; pigments contents; number and shape of stomata; mineralsconcentrations; isozymes; and SDS -PAGE protein profile in Pisum sativum plant at 36 days under drought and ABAtreatments. The results showed that ABA reduces the reduction in both fresh and dry weights shoot and root of Pisuminduced by drought stress. Proline content was increased in response to drought and ABA treatments. Water-stress causedsignificant reduction in Chl. a, b, whereas application of ABA significantly increased in total chlorohpyll. Water stresssignificantly decreased IAA, GA concentrations and increased ABA level in leaves than these of the control. ABA treatmentpartially overcame the decrease in IAA and GA contents. Water stress and ABA increased the activity of (POD) and (ACP)enzymes in Pisum plants. Proteins profile of Pisum sativum in shoot revealed qualitative and quantitative changes, alsoappearance or disappearance of some bands. After 54 days the yield harvested and total carbohydrates and SDS -PAGEprotein profile in seed were investigated. The data showed that total carbohydrates contents were significantly decreased inplant under drought and ABA partially alleviate the adverse effect of water-deficit stress.