PRELIMINARY COMPARATIVE ANALYSIS OF FOUR BOTANICALS USED IN THE TRADITIONAL MEDICINES OF PAKISTAN

摘要

Jordan is unanimously considered to be one of the centers of genetic diversity for barley, where wild and landraces ofbarley has been grown under different climatic conditions. The genetic diversity and genetic structure based on hordeinpolymorphism was assessed in 90 different accessions collected from four different sites of Jordan. A-PAGE was used toreveal hordein polymorphism among the genotypes. A total of 29 distinct bands were identified, out of them 9 bands weredistinguished for D, 11 for C, and 9 for the B hordein regions. The observed genetic similarity was an exceptionally highbetween the populations than expected, which is probably due to high gene flow estimated between them. The geneticdiversity parameters were not differ largely among the populations, indicating that local selection of a particular site did notplay a key role in shaping genetic diversity. Analysis of molecular variance (AMOVA) revealed significant populationstructure when accessions were structured according to population site. There was 94% of hordein variation resided withinthe populations and only 8% present among the populations. Both Bayesian and Principale Coordinate Analysis (PCoA)concordantly demonstrated admixture genotypes of the landraces barley populations. Consequently, none of the populationfound to be clustered separately according to its population site. It is concluded that this approach can be useful to explorethe germplasm for genetic diversity but perhaps is not suitable for determining phylogenic relations in barley.