EVALUATION AND SCREENING OF RESISTANCE TO REPLANT IN GERMPLASM OF GRAPE AND PHYSIOLOGICAL MECHANISMS OF ITS RESISTANCE

摘要

Assessment of genotoxins-induced DNA damage and mutations at molecular level is important in ecogenotoxicology.Treatment with Peganum harmala crude extract can result in cytotoxic, carcinogenic and mutagenic effects. Mutagenesis isindicative of genetic instability and can be assayed using microsatellites or simple sequence repeats (SSRs). The application ofSSR marker analysis using capillary electrophoresis (CE) apparatus showed differences in SSR profiles within the extract aswell as between the two extracts. All the 12 SSR primer pairs used produced amplification products which showed 100%polymorphism. The total number of scoreable loci obtained with the 12 used primers were 112 alleles. Of the 112 allelesdetected 12 alleles (10.7 %) were considered as zero alleles. Three alleles produced by various primers were detected at locusVfG_22 to 15 at locus VfG_693 and VfG_873, with an average of 9.3 bands/SSR. The discrimination power of each SSR locuswas estimated by the PIC, which ranged from 0.47 for locus VfG_22 to 0.88 for locus VfG_693 with a mean of 0.70. About83.3% of the 18 alleles observed in untreated samples using the 12 primers disappeared from almost all the treatments withwater and ethanol extracts. Statistical analysis of morphometric traits showed significant differences between the two extractsin all studied traits except the percentage of germination and root dry weight which were non significant. However, on the otherhand significant differences were observed between treatments and the control for all the traits studied.