ESTABLISHMENT OF AN EFFICIENT AND REPRODUCIBLE REGENERATION SYSTEM FOR POTATO CULTIVARS GROWN IN PAKISTAN

摘要

The present study was carried out to assess the effect of growth regulators in three different combinations on masspropagation of currently grown three potato cultivars cv. Desiree, Kuroda and Cardinal. Varietal response on In vitroregeneration under different hormonal combinations. For callus induction, internodes of potato cultivars were cultured onmodified Murashige & Skoog (MS) medium, added with different growth hormonal combinations. Callusing frequency forall these treatments and cultivars were recorded and in callus induction medium (CIM1) explants showed significantlyhigher callus formation as compared to two other combinations. For shoot induction, calli were cultured on Murashige &Skoog (MS) modified medium, supplemented with different hormonal combinations. Shoot induction medium (SIM3) gavebest shoot induction frequency as compared to other media combinations. On the same media, an average number of shootsper explant were obtained for cultivar Desiree which is significantly different from the other two media combinations.Overall, the In vitro regeneration and multiplication potential was highest in the variety Cardinal followed by Kuroda andDesiree. The interaction between different hormonal combinations and varietal response for all the parameters showedsignificant differences.