EFFECTS OF SALICYLIC ACID ON THE GROWTH AND PHYSIOLOGICAL CHARACTERISTICS IN CYCLOCARYA PALIURUS SEEDLINGS

摘要

Field studies were conducted to examine the effects of salicylic acid (SA) on the growth and physiologicalcharacteristics of Cyclocarya paliurus seedlings by spraying the foliage with 0.0 (control), 0.2, 1.0, and 2.0 mMsalicylic acid (SA). Proper concentrations of SA improved the relative growth yield of seedling stems and thesoluble protein and sugar content of the leaves. It also increased the content of secondary metabolites includingtriterpenoids, flavonoids, quercetin and kaempferol, mineral elements K, Ca, Mg, Mn, Fe and Cu. Moreover, itstimulated the activities of superoxide dismutase (SOD), peroxidase (POX) and phenylalanine ammonia-lyase(PAL) in the leaves. The effects of SA on these indices were dose dependent. The relative growth of seedlingstem diameter and quercetin content increased gradually with an increase in concentration of SA from 0.0-2.0mM. A concentration of 0.2 mM was optimal to promote content of soluble protein, sugars, Ca, Mg, Mn, Fe,and Cu, and SOD activity and significanty increased by 38.6%, 22.1%, 17.7%, 8.2%, 20.3%, 23.2%, 15.6%,and 52.4%, respectively, as compared with the control (CK). However, the maximal increase in activities ofPAL, POX, and content of triterpenoids, kaempferol, and flavonoids was attained at 1.0 mM treatment, whichsignificantly increased by 76.5%, 78.4%, 76.4%, 96.3%, and 107.4%, respectively, compared with CK.Correlation analysis revealed positive relationships between activities of PAL, POX and content oftriterpenoids, quercetin, kaempferol, and flavonoids within a certain concentration range of SA. These resultssuggested that an appropriate concentration (0.2-1.0 mM) of SA was not only effective in the improvement ofphysiological function of C. paliurus, but also increased seedling resistance; additionally, it helped to stimulatethe synthesis of medicinal components in leaves．