Clinical Research on the Method of Huafu Zaisheng in Treating Diabetic Foot with Tendons Necrotic

摘要

Objective: To observe the effect of liquefying the necrotic tendons of diabetic foot and promotion of wound healing using the treatment of Huafu Zaisheng. Methods: 98 patients suffering from diabetic foot were randomly divided into Huafu Zaisheng group (35 cases), Huafu group (32 cases), Zaisheng group (31 cases), and then had eroded debridement timely for its wounds. Since then, different methods and drugs applied to deal with the wound, observe the liquefaction time and detachment time of the degeneration and necrosis tendon, and observe the wound healing. Collection and preservation of wound secretion, determine the pH value of wound secretion, determination content of the NO and TGF- β 1 in wound secretion. Wound tissue containing fascia and tendons was extracted by surgical methods, before treatment and 6, 12 weeks after treatment. All tissue samples were prepared according to Hematoxylin-eosin staining techniques, after which their appearance was evaluated for pathological changes using an optical microscope. Immunohistochemistry staining was used to detect the expressions of CD34 and CK19. Results: By comparison of the efficacy of the three groups, the curative effect of the HFZS group is superior to HF and ZS group. The necrotic tendons of Huafu Zaisheng group were liquefied clearer than the other two groups. Three groups of secretions pH value were the trend of first decreased, then increased, weakly basic first, and then weak acid, and then close to neutral. Compared to ZS group and HF group, the difference was statistically significant ( P < 0.01). After 4.6 weeks’ treatment, compared to ZS group and HF group, the NO, TGF- β 1 content of HFZS group increased significantly, and the difference was statistically significant ( P < 0.01). Conclusion: Using the method of Huafu Zaisheng, the necrotic tendons and tissues could be liquefied as early as possible, and then promote the wound healing. Application of Huafuzaisheng method to deal with wounds, can remove necrotic tendons and fascia tissue as early as possible, can change the wound pH value and provide a variety of active factors (NO, TGF- β 1) and microenvironment for wound healing.