Patogenicidade de Verticillium lecanii ao pulg?o-do-pinus

摘要

The objective of this work was to evaluate the pathogenicity of the entomopathogenic fungus Verticillium lecanii isolate IBCB 473 to the aphid Cinara atlantica (Hemiptera: Aphididae), insect pest in Pinus spp plantations. The trials were conduced in a completely randomized design, treatments with 5 repetitions, and each repetition consisting of one Petri dish (9 cm of diameter) containing 10 nymphs. Suspensions adjusted to concentrations of 1,0 ' 106; 0,5 ' 107; 1,0 ' 107; 0,5 ' 108 e 1,0 ' 108 conidia/mL were tested by spraying 1mL over the nymphs. Sterilized water was used as control treatment. The Petri dishes were maintained at 25±1°C, 70±10% RH and 12-hour photophase. The evaluations were carried out daily, recording the individual mortality of each treatment. The best concentration was 1,0 x 108 conidia/mL with mortality of 86,0% after five days. LT50 for each concentration was 5,82; 5,24; 4,60; 4,34 and 3,83 days, respectively.