ANNONA MURICATA FRUIT MEDIATED BIOSYNTHESIS, PHYSICOCHEMICAL CHARACTERIZATION OF MAGNETITE (Fe3O4)NANOPARTICLES AND ASSESSMENT OF ITS IN VITRO ANTIDIABETIC ACTIVITY

摘要

The biological preparation of magnetite (Fe3O4) nanoparticles (NPs) plays a vital role in the nanotechnology field. In the current study, we report here the preparation and physicochemical characterization of Fe3O4 nanoparticles by using Annona muricata fruit source, which acted as reducing material in the nanoparticle preparation. UV-Visible spectra of magnetite nanoparticles showed a peak at 340 nm due to the SPR. The Fourier transform infrared spectra indicate a very intense absorption band at 657cm-1, which proved the existence of magnetite in the prepared material. The crystal size of magnetite NPs was calculated using an X-ray diffractometer and it was estimated to be 23nm; the prepared nanoparticles are cubic in structure. The surface morphology, crystalline purity and magnetic property were examined by SEM-EDX and VSM. The synthesized nanoparticles were examined for their antidiabetic strength by α-Amy inhibitory activity. Acarbose was used as a pharmacological inhibitor. The inhibitory potential of prepared nanoparticles against alpha-amylase proves their therapeutic role.