Longitudinal analysis of subtype C envelope tropism for memory CD4+ T cell subsets over the first 3?years of untreated HIV-1 infection

摘要

BACKGROUND:HIV-1 infects a wide range of CD4sup+/sup T cells with different phenotypic properties and differing expression levels of entry coreceptors. We sought to determine the viral tropism of subtype C (C-HIV) Envelope (Env) clones for different CD4sup+/sup T cell subsets and whether tropism changes during acute to chronic disease progression. HIV-1 envs were amplified from the plasma of five C-HIV infected women from three untreated time points; less than 2?months, 1-year and 3-years post-infection. Pseudoviruses were generated from Env clones, phenotyped for coreceptor usage and CD4sup+/sup T cell subset tropism was measured by flow cytometry.RESULTS:A total of 50 C-HIV envs were cloned and screened for functionality in pseudovirus infection assays. Phylogenetic and variable region characteristic analysis demonstrated evolution in envs between time points. We found 45 pseudoviruses were functional and all used CCR5 to mediate entry into NP2/CD4/CCR5 cells. In vitro infection assays showed transitional memory (TM) and effector memory (EM) CD4sup+/sup T cells were more frequently infected (median: 46% and 25% of total infected CD4sup+/sup T cells respectively) than na?ve, stem cell memory, central memory and terminally differentiated cells. This was not due to these subsets contributing a higher proportion of the CD4sup+/sup T cell pool, rather these subsets were more susceptible to infection (median: 5.38% EM and 2.15% TM cells infected), consistent with heightened CCR5 expression on EM and TM cells. No inter- or intra-participant changes in CD4sup+/sup T cell subset tropism were observed across the three-time points.CONCLUSIONS:CD4sup+/sup T cell subsets that express more CCR5 were more susceptible to infection with C-HIV Envs, suggesting that these may be the major cellular targets during the first 3?years of infection. Moreover, we found that viral tropism for different CD4sup+/sup T cell subsets in vitro did not change between Envs cloned from acute to chronic disease stages. Finally, central memory, na?ve and stem cell memory CD4sup+/sup T cell subsets were susceptible to infection, albeit inefficiently by Envs from all time-points, suggesting that direct infection of these cells may help establish the latent reservoir early in infection.