Effectiveness of Garlic Bulb (Allium Sativum L.) Ethanol Extract Cream on the Number of Fibroblast Cells in the Healing Process of Male White Rat (Rattus norvegicus L.) Wistar Strain Incision Wound

摘要

Background: The case of antibiotic resistance in the standard treatment of skin wounds is now a major problem in the medical world, thus herbal preparations are needed as an alternative. However, wound healing creams that are currently available on the market are the result of extraction from Centella asiatica whose insignificant antibacterial and anti-inflammatory activity. Therefore, in this research, another alternative herbal medicine cream from garlic bulb (Allium sativum L.) extract is being examined to see its effectiveness in healing incision wounds. Methods: The following method is used by the researchers to analyze the effectiveness of topical preparations of garlic bulb ethanol extract on the increase of fibroblast cells in the healing process of incision wounds. This research was conducted in June - August 2019 by scraping incision in the back area of male white rats (Rattus norvegicus L.) Wistar strain with a size of ± 1 cm and a thickness of ± 2 mm. Furthermore, a true experimental design was carried out using a post-test control group design using 5 treatment variables for 7 days into 5 groups of mice randomly selected. The skin tissue that has been given treatment is then extracted and painted using Hematoxylin-eosin (HE) to then be observed the number of fibroblast cells under a microscope with a magnification of 400 times at 5 times the field of view. The data were analyzed using the Post Hoc test which then summarized using the Mann Whitney test (p <0.05). Results: There is an overall difference in the concentration of garlic bulb (Allium sativum L.) ethanol extracts on the increase of fibroblast cells for the incision wound healing process of the male white rats (Rattus norvegicus L.) Wistar strain. Conclusion: The results of histopathological observations using fibroblast cell scoring can be concluded that the concentration groups of 20% and 15% have the highest scoring values of 3 with "moderate" interpretation.