High‐quality draft genome sequence of Gaiella occulta isolated from a 150 meter deep mineral water borehole and comparison with the genome sequences of other deep‐branching lineages of the phylum Actinobacteria

摘要

Gaiella occulta strain F2‐233 T (=CECT 7815?=?LMG 26412), isolated from a 150 meter deep mineral water aquifer, was deemed a candidate for high‐quality draft genome sequencing because of the rare environment from which it was isolated. The draft genome sequence (QQZY00000000) of strain F2‐233 T is composed of approximately 3?Mb, predicted 3,119 protein‐coding genes of which 2,545 were assigned putative functions. Genome analysis was done by comparison with the other deep‐branching Actinobacteria neighbors Rubrobacter radiotolerans , Solirubrobacter soli and Thermoleophilum album . The genes for?the tricarboxylic acid cycle, gluconeogenesis and pentose phosphate pathway, were identified in G. occulta , R. radiotolerans , S. soli and T. album genomes. Genes of the Embden–Meyerhof–Parnas pathway and nitrate reduction were identified in G. occulta , R. radiotolerans and S. soli , but not in the T. album genome. Alkane degradation is precluded by genome analysis in G. occulta . Genes involved in myo ‐inositol metabolism were found in both S. soli and G. occulta genomes. A Calvin–Benson–Bassham (CBB) cycle with a type I RuBisCO was identified in G. occulta genome, as well. However, experimental growth under several conditions was negative and CO 2 fixation could not be proven in G. occulta .