首页> 外文期刊>Microbiology >The 52°-55° segment of the Bacillus subtilis chromosome: a region devoted to purine uptake and metabolism, and containing the genes cotA, gabP and guaA and the pur gene cluster within a 34960 bp nucleotide sequence
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The 52°-55° segment of the Bacillus subtilis chromosome: a region devoted to purine uptake and metabolism, and containing the genes cotA, gabP and guaA and the pur gene cluster within a 34960 bp nucleotide sequence

机译:枯草芽孢杆菌染色体的52°-55°段:致力于嘌呤摄取和代谢的区域,并在34960bp核苷酸序列中含有基因COTA,GABP和GUAA和PUR基因簇

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摘要

Within the framework of the international project for sequencing the entire Bacillus subtilis genome, we have determined the complete sequence of the segment flanking the purE-D gene cluster (55°) as far as cotA (52°). This segment (34960 bp) contains, as well as 12 genes already identified as part of the pur operon, 17 putative ORFs and one partial one. Two of them (gabP and guaA) are known B. subtilis genes. The gene product of cotA (formerly pig) shows significant similarity to oxidoreductases (phenoxazine synthase and bilirubin oxidase). The putative products of ORFs yeaB (Czd protein), yeaC (MoxR), yebA (CNG-channel and cGMP-channel proteins from eukaryotes), yebB (hypothetical 32.9 kDa protein of Escherichia coli), yecA (amino acid permease) and yecB (adenine deaminase) were similar to proteins in data banks.
机译:在国际芽孢杆菌基因组测序的国际项目框架内,我们已经确定了纯-d基因簇(55°)的完整序列(55°),至于COTA(52°)。该段(34960bp)包含,以及已经被识别为PUR操纵子,17个推定ORF和一个部分的基因。其中两个(GABP和GUAA)是已知的B.枯草芽孢杆菌基因。 COTA(以前猪)的基因产物与氧化还原酶(吩恶嗪合酶和胆红素氧化酶)显示出显着的相似性。 ORFS YEAB(CZD蛋白),YEAC(MOXR),YEBA(CNG通道和来自真核生物CGMP型蛋白质)的推定产品,YEBB(大肠杆菌的假设32.9kDa蛋白),YECA(氨基酸允许)和YECB(腺嘌呤脱胺酶类似于数据库中的蛋白质。

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