Long non-coding RNA HANR as a biomarker for the diagnosis and prognosis of colorectal cancer

摘要

Previous work suggests that the long noncoding RNA HCC associated long non-coding RNA (HANR) is associated with hepatocellular carcinoma (HCC) progression, but its significance in the context of colorectal cancer (CRC) remains to be determined. Therefore, in this study we assessed the prognostic and diagnostic value of HANR in patients suffering from CRC. The HANR expression in 165 pairs of CRC cancer and adjacent non-cancerous prostate tissues was measured by quantitative reverse transcription polymerase chain reaction (qRT-PCR) analysis. Student t test was conducted for intergroup comparison. Pearson correlation test was used for correlation analysis. Survival curves were carried out by the Kaplan-Meier method and evaluated using the log-rank test. Multivariable Cox proportional hazard risk regression model was performed to screen the independent factor affected the prognosis of CRC patients. In this study, levels of HANR were significantly higher in CRC tumor samples relative to adjacent normal tissue samples ( P .001). A ROC analysis suggested HANR expression could be reliably used to differentiate between normal and CRC tumor tissue. In addition, elevated HANR expression was positively correlated with more advanced and aggressive CRC features, such as a larger tumor size ( P = .003), increased invasion depth ( P = .012), and more advanced TNM stage ( P = .011). Survival analyses revealed that elevated HANR expression was correlated with worse overall survival ( P = .002) and disease-free survival ( P = .003). A multivariate analysis further confirmed the relevance of HANR as an independent predictor of CRC patient outcomes. In summary, these results indicate that the lncRNA HANR is a promising prognostic indicator in CRC patients.