INCIDENCE AND MOLECULAR CHARACTERISATION OFVIRUSES INFECTING WHEATIN EASTERN ANATOLIA (TURKEY)

摘要

In order to assess the occurrence and the inci-dence of Barley yellow d w a r f viruses (BYDV-PAV, BYDV-SGV, CYDV-RPV, BYDV-MAV, BYDV- RMV), Wheat streak mosaic -virus (WSMV) and Soilborne wheat mosaic irus (SBWMV) in eastern An-atolia, a survey was conducted in wheat fields from 10 major wheat-growing provinces in Turkey from April 2012 to June 2013. A total of 900 wheat sam-ples were collected and reverse-transcription poly-merase chain reaction (RT-PCR) and multiplex RT- PCR tests were implemented to detect seven wheat viruses. In wheat fields, BYDV-PAV was the most common, followed by BYDV-SGV, CYDV-RPV and WSMV, respectively. BYDV-MAV, BYDV- RMV, and SBWMV were not detected in tested sam-ples. Since the SBWMV has been transmitted by soil borne fungus P olymyxa graminis, further research was performed to determine the presence of SBWMV and its fungus vector in soil samples by bait plant method. None of the green parts and roots of the bait plants were found infected by SBWMV and P. graminis, respectively. In the wheat fields of eastern Anatolia, total virus incidence ranged from 0.4 to 6.8%. Based on field observations, the inci-dence of surveyed viruses in all wheat fields was less than 7 %. Since detected viruses have been consid-ered the most economically relevant viruses of wheat fields, we further characterized randomly selected isolate of each detected virus by cloning the com-plete coat protein gene and sequencing [(Accession numbers; BYDV-PAV (KC900900), CYDV-RPV (KC900903), BYDV-SGV (partial) (KC900899) and WSMV (KC900901)]. Nucleotide sequence identities for the coat protein gene (CP) ranged from 82 % to 94 % (PAV), 69 % to 96% (SGV), 83% to 96 % (CYDV-RPV), and from 88 % to 97 %(WSMV). This is the first report of' BYDV-PAV, BYDV-SGV, CYDV-RPV and WSMV infecting wheats in eastern Anatolia (Turkey).