A First NGS Investigation Suggests No Association Between Viruses and Canine Cancers

摘要

Approximately 10-15% of worldwide human cancers are attributable to viral infection. When operating as carcinogenic elements, viruses may act with various mechanisms, but the most important is represented by viral integration into the host genome, causing chromosome instability, genomic mutations and aberrations. In canine species, few reports have described an association between viral integration and canine cancers, but more comprehensive studies are needed. The advancement of next generation sequencing (NGS) and the cost reduction have resulted in a progressive increasing of sequencing data in veterinary oncology offering an opportunity to study virome in canine cancers. In this study, we have performed viral detection and integration analyses using VirusFinder2 software tool on available whole genome (WGS) and whole exome sequencing (WES) data of different canine cancers. Several viral sequences were detected in lymphomas, hemangiosarcomas, melanomas and osteosarcomas, but no reliable integration sites were identified. Even if with some limitations such as the depth and type of sequencing, a restricted number of available non-human genomes software, and a limited knowledge on endogenous retroviruses in the canine genome, results are compelling. However, further experiments are needed and similarly to feline species dedicated analysis tools for the identification of viral integration sites in canine cancers are required.