COMPARISON EFFECT OF BONE MARROW DERIVED MESENCHYMAL STEM CELLS AND STIMULATED BONE MARROW MESENCHYMAL STEM CELLS WITH LPS ON HEALING OF INDUCED THIRD-DEGREE SKIN BURN IN MOUSE

摘要

Background &?Aims : Burning provides irreparable effects on the affected patient. Several studies show that these cells may contribute to tissue regeneration whether through producing a variety of bioactive growth factors and/or by differentiation into mesoderm lineage. Several studies demonstrate that stimulated mesenchymal stem cells have more therapeutic potential than unstimulated cell in accelerate tissue wound healing. The aim of the present study was to investigate the effect of subcutaneous administration of stimulated bone marrow-derived mesenchymal stem cells with lipopolysaccharide in repairing or regeneration of skin wounds induced by third-degree burn and to compare it with unstimulated Mesenchymal stem cell in mouse model. Materials & Methods : The third-degree skin burn was induced on male mice (N=27). After 1 hour, based on the equal physical condition mice were randomly divided into three separate groups which included control, mesenchyme and LPS groups and then respectively subcutaneously administered with phosphate buffered saline (PBS; 400 μl), mesenchymal stem cells and stimulated mesenchymal stem cells with LPS (106 cell in 400μl PBS) at the burn site. In order to check the speed of the process of 21-days wound repair, tissue section at days 7, 14, and 21 after induction of burn were prepared and stained with hematoxylin/eosin?and?Masson’s trichrome. Results : Considering investigated parameters including formation of granulation tissue, angiogenesis, fibroblast proliferation, collagen deposition and the rate and quality of healing of third-degree thermal burns were significantly accelerated in mesenchyme and LPS groups when compared to the control group (P0.05). As well as LPS group in comparison to mesenchymal group was superior in collagen deposition and rate of wound healing. Conclusion : The results demonstrate that stimulate cells whit LPS improve BMSc?s therapeutic effect in some factors of burn wound healing.