QUALITY INDICES OF WATERS FLOWING AWAY FROM CATCHMENTS OF SMALL RETENTION RESERVOIRS PLANNED IN THE KRAKOW REGION

摘要

Flow cytometry (FC) was used for measuring ploidity and number of cells at each phase of the cell cycle of four Yarrowia lipolytica yeast strains. Synchronization at G0/G1-phase was partially achieved by carbon source starvation, followed by glucose addition. Approximately 10 000 cells were analyzed by measuring the fluorescence of propidium iodine (PI) bound to DNA after 0, 1, 2, 4, 6 hours of glucose addition to the starved cells population. Estimation of cells percentage at each cycle phase was achieved by Cylchred and WinMDI analysis of obtained data. However complete synchronization was not obtained for any of the strains, the generation time representing time interval between two maxima of cells at S phase was determined. The shortest generation time was noted for Y. lipolytica AWG7 (1h).Comparison of histograms of all tested strains with a haploid type strain proved their haploidy.