Expression analysis, single-nucleotide polymorphisms of the Myoz1 gene and their association with carcase and meat quality traits in chickens

摘要

Previous studies reported that the Myoz1 gene plays a crucial role in signal transduction andmuscle fibre type differentiation. This finding suggests that the Myoz1 gene is a potential candidate for affecting carcase and meat quality traits in animals. Therefore, this study aimed todetect genetic variations in Avian and Yellow Bantam chickens and to evaluate the effects ofthese variations on economically important carcase and meat-quality traits. Expression analysisusing quantitative real-time PCR indicated that Myoz1 was broadly expressed in all four studiedtissues (liver, heart, breast muscle and leg muscle). Expression of Myoz1 was significantly higherin both leg muscle and breast muscle compared to the liver and heart. Then, using directsequencing, a total of five single nucleotide polymorphisms (SNPs) were identified within theMyoz1 gene in two breeds of chickens. These breeds included three non-synonymous SNPs(SNP1: g. 16022512 G T, SNP3: g. 16022560 C T and SNP5: g.16023903 A G) correspondingto A103S, H119Y and S189G, respectively. Statistical analyses indicated that SNP2 (g. 16022529T C), SNP3, SNP4 (g.16023878 A C) and SNP5 significantly influenced some carcase and meatquality traits. Meanwhile, we did not find any association between the constructed haplotypeand carcase traits in the Avian nor Yellow Bantam chickens. In conclusion, SNPs in the Myoz1gene could be used for marker-assisted selection in chicken breeding.