Left ventricular phosphorylation patterns of Akt and ERK1/2 after triiodothyronine intracoronary perfusion in isolated hearts and short-term in vivo treatment in Wistar rats

摘要

Objective(s): To determine the effects of triiodothyronine (T3) intracoronary perfusion in isolated hearts and short-term administration in rats on the left ventricular (LV) phosphorylation patterns of Akt and ERK1/2. Materials and Methods: Cardiodynamic and hemodynamic parameters were evaluated in Langendorff–perfused hearts. Left ventricles were used for histomorphometric and Western blot analyses. Short-term hyperthyroidism was established by T3 (500 μg.kgsup-1/sup.dsup-1/sup; subcutaneous injection) for 1 (T3sub1d/sub), 3 (T3sub3d/sub), and 10 (T3sub10d/sub) days. Results: Isolated hearts receiving T3 perfusion did not modify LV developed pressure, +dP/dtsubmax/sub, -dP/dtsubmin/sub, heart rate, and coronary perfusion pressure compared with vehicle-perfused hearts. P-ERK1/2 and p-Akt levels in LV tissues after 5, 15, or 60 min of T3 or vehicle perfusion were similar. Compared with their time-matched controls, isolated hearts of T3sub3d/sub and T3sub10d/sub rats exhibited LV hypertrophy and increased absolute values of +dP/dtsubmax/sub and -dP/dtsubmin/sub (i.e., positive inotropic and lusitropic effects). P-ERK1/2 decreased in LV tissues of T3sub1d/sub and T3sub10d/sub but not in those of T3sub3d/sub rats, and p-Akt levels augmented in left ventricles of T3sub3d/sub and stayed unaltered in those of T3sub1d/sub and T3sub10d/sub rats. Conclusion: T3 intracoronary perfusion did not alter cardiodynamics and hemodynamics nor influence the activation of Akt and ERK of normal hearts. Accordingly, the rapid non-genomic effects of T3 were not evident. Short-term T3 treatment provoked cardiac hypertrophy coincidental with increased LV function and associated with transient Akt activation and cyclic ERK1/2 inhibition; which implies activation of physiological hypertrophy signaling and deactivation of pathological hypertrophy signaling, respectively.