ACUTE DERMAL IRRITATION STUDY OF POLYHERBAL GEL MASTILEP IN RABBITS

摘要

Objective: The current study was designed to study Acute Dermal Irritation potential of Mastilep gel (supplied by M/S Ayurvet Limited, Baddi, India) according to OECD guidelines. Materials and Methods: Mastilep gel, a new herbal ointment for topical use applied on the udder, has been developed as an alternative method for controlling mastitis in ruminants. Mastilep gel’s active ingredients including herbal extracts of Cedrus deodara, Curcuma longa, Glycyrrhiza glabra and Eucalyptus globulus are expected for their antibacterial, anti-inflammatory, analgesic, antihistaminic and immunomodulatory effects. For new substances it is the recommended stepwise testing approach for developing scientifically sound data on the corrosivity/irritation of the substance. Three female rabbits were used for the study. Each animal served as its own control. After application of Mastilep gel the degree of irritation/corrosion was read and scored. Results: No severe erythema, oedema or any skin lesion was observed after Mastilep gel application. The results revealed no irritation potential of Mastilep gel. Conclusion: MASTILEP gel is safe for usage