Molecular characterization and distance tree identification of unique environmental carryover proteobacteria Mesorhizobium sp. in the midgut of Anopheles stephensi

摘要

Recently, environmental carry over symbiotic bacteria has a significant attention in the role of microbesin mosquito gut. The gut microbes influence the transmission and development of pathogens. However,the effects of micro biota in guts made the parasites to proliferate with the host interaction. Soidentification of gut microbe is very important for vector control mainly on malarial mosquitoes.Anopheles stephensi is a primary vector for spreading malarial disease in Asia and most predominant inthe Kancheepuram district, Tamilnadu, India. In this present investigation we found that uniqueenvironmental carryover soil Proteobacteria in midgut of Anopheles stephensi where malaria feveroutbreak subtropical zone. Larvae of Anopheles stephensi mosquitoes were collected from kancheepuramdistrict, Tamilnadu, India. The genomic DNA isolated and 16s rRNA gene sequencing performed forbacterial identification in midgut region of Anopheles stephensi mosquitoes and distance tree constructedto determine the origin. Our finding suggested that single existence of soil environmental MesorhizobiumSp. in the gut. Most of the other bacterial genera in the study have been already identified. Scientistswere under pressure to investigate further to know mechanism of the carryover environmental microbesto mosquitoes. This preliminary interesting report gives a path for the scientist to explore further tocontrol the malarial vector disease.