Detection of KDR l1014f mutation in pyrethroids susceptible Anopheles gambiae S.L from Ladanai, Kano state, northwest Nigeria

摘要

Insecticides resistance has been a major challenge in malaria control effort worldwide. Understandingspecie composition and their resistance mechanisms towards the major insecticides for vector control willgreatly contribute in overcoming these phenomena. Larvae of anopheles mosquitoes collected fromLadanai, Nasarawa Local Govt, were reared to adults, after morphological identification of An gambiaes.l and their resistance profile, DNA extracts were used for determination of molecular speciecomposition and detection of kdr 1014F mutation. The result of the study shows that An gambiae s.l, themajor malaria vector in the study site where highly resistant to pyretheroids permethrin and delatmethrininsecticides. Although some traces of An gambiae ss and An arabiensis were detected, An colluzii is themajor molecular form specie identified in the site. High frequency of Kdr 1014F mutation was detectedin phyretheroids susceptible An gambiae ss followed by An colluzii and least in An arabiensis. Thefinding of this study established insecticides resistance in the study site which may be associated withpresence of KDR 1014F mutation triggered by indiscriminate use of insecticides for residential andagricultural purposes. Establishment of Insecticides resistance in the study site and predominance of Ancolluzii coupled with high frequency of Kdr 1014F mutation can guide the stake holders in implementingthe most appropriate measures of vector control strategy in the study area.