Phylogeny of Korean Hornbeam (Carpinus turczaninovii) based on nuclear ribosomal ITS sequence

摘要

The genus?Carpinus?belonging to Coryloideae, Betulaceae, has significant economic and ornamental importance. This study?was undertaken with?the?aim to understand the genetic diversity among eighteen isolates of?Carpinus turczaninovii?collected from different geographical regions of Korea, using ribosomal RNA (rRNA) internal transcribed spacer (ITS) sequences, to compare the infraspecific-phylogenetic relationships among?C. turczaninovii?in Korea, and some known?Carpinus?plants. The size variation of sequenced rRNA ITS regions was not seen, with 215, 162, 222 bp of ITS1 region, 5.8S rRNA gene, ITS2 region, respectively. However, some certain nucleotide variations resulted in genetic diversity. In the genus?Carpinus,?C. turczaninovii?closely genetic with?Castanopsis?kawakamii,?Carpinus orientalis,Carpinus monbeigiana, and?Calyptranthes?polynenra?formed one monophyletic clade, while?Carpinus betulus?and?Carpinus laxiflora,?respectively formed one monophyletic clade. In the present ITS sequence analysis,?Carpinus fangiana,Carpinus coreana,?Carpinus japonica, and?Carpinus caroliniana?were considered as Out Group, compared to?C. turczaninovii?group. The highest intraspecific variation was found between?C. caroliniana?and?C. japonica. The least intraspecific variation was obtained between?C. turczaninovii?and?C. coreana. The results will help further understanding?Carpinus?infraspecies population and their phylogenetic analysis.