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Aluminum elicitation improves antioxidant potential and taxol production in hazelnut (Corylus avellana L.) cell suspension culture

机译:铝合金诱导在榛子(Corylus Avellana L.)细胞悬浮培养中提高抗氧化潜力和紫杉醇生产

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Taxol was originaly found in yew (Taxus sp.) but it also ocurrs in other species like hazelnut (Corylus avellana L.). We investigated production of taxol in suspension cultures of hazelnut following elicitation by aluminium chloride supplemented at 0, 25, 50 and 100 µM concentration. Aluminium elicitation has a significant effect decreasing cell growth and protein content but increasing production of taxol, total phenolics, anthocyanin and flavonoids. It also increases antioxidant potential and other antioxidant system components such as proline and enzymes like catalases and peroxidases. Additionally, aluminium increased hydrogen peroxide and malondialdehyde content of cells. Apart from taxol production aluminium improved its release into the medium and total yield in comparison to controls. In general, for the increased taxol synthesis application of 50 and 100 µM of aluminium elicitor in cell culture provided acceptable results.
机译:紫杉醇是在伊斯法(Taxus sp.)中发现的原始原味,但它也像榛子(Corylus avellana L)这样的其他物种。通过补充0,25,50和100μM浓度的氯化铝,研究了榛子溶液悬浮培养物中的产量。铝合金闪导具有显着效果降低细胞生长和蛋白质含量,但增加了紫杉醇,总酚类,花青素和黄酮类化合物的产生。它还增加了抗氧化潜力和其他抗氧化体系统组分,例如脯氨酸和酶,如过氧化氢酶和过氧化物酶。另外,铝增加过氧化氢和细胞的丙二醛含量。除了紫杉醇生产铝外,还将其释放到培养基中,与对照相比,总收率。通常,对于增加的紫杉醇合成施用50和100μM铝培养物中的铝素提供可接受的结果。

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