Direct determination of piroxicam in pharmaceutical forms using flow injection- spectrophotometry

摘要

In this research, direct batch and flow injection (FI) spectrophotometric methods for the analysis of piroxicam (PIX) in commercial dosage forms were investigated. The methods were based on the reaction of PIX with diazotized metoclopramide in alkaline medium to form orange color product at room temperature which absorbs maximally at λmax 472 nm. Chemical and physical variables of batch and FI methods were optimized to produce high sensitivity and reproducibility. Under the optimized experimental conditions, Beer’s law was obeyed over concentration ranges from 1 - 35 and 10 - 250 μg/mL PIX with limits of detection of 0.2 and 3.4 μg/mL and limits of quantification of 0.7 and 11.4 μg/mL PIX for batch and FI procedures, respectively. The relative standard deviation (%RSD) was less than 0.7 and 2.4 for batch and FI procedures respectively with a sample throughput of 38 h-1 for FI procedure. The proposed methods can be used for the routine analysis for the assay of PIX in injections and capsules.