Background The purpose of the present investigation was to clarify the impact of the treatment of interruptions on the durations and the frequency of the physical activity (PA) bouts under free-living conditions. Methods One hundred and forty adults (50?±?7 years) wore an accelerometer (Lifecorder) for seven consecutive days under free-living conditions. According to the minutes by minutes metabolic equivalents (METs) value, the PA was divided into one of three intensity categories: light intensity PA (LPA, 6 METs), and the sum of the MPA and VPA was defined as moderate to vigorous intensity PA (MVPA, > 3 METs). Thereafter, based on the time series data, we defined MVPA bouts as PA that was maintained at no less than 3 METs completely for 10 minutes or longer with or without allowing for a one-minute or a two-minute break ( Results The frequency and duration of the continuous MVPA bouts lasting longer than 10-min were significantly lower and shorter compared with that in the non-continuous MVPA bouts allowing a one- or two-minute interruption (4.11?±?1.65, 6.58?±?2.72 and 8.97?±?3.55 bouts/day, 71.62?±?33.66, 119.03?±?49.35 and 169.75?±?65.87 min/day, P? Conclusion The treatment of interruptions for the setting of the accelerometer affects the estimation of the MVPA bouts under free-living conditions in middle-aged to older adults. The best analysis process with regard to the accelerometer quantifying the break to reflect the real behavioral pattern and the physiological stress in such subjects remains unclear.
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