Isolation of secondary metabolites from the mediterranean sponge species; Hemimycale columella and its biological properties

摘要

Despite the richness and biodiversity of invertebrates and algae in the Mediterranean Sea, these organisms are stillpoorly studied. The objective of our research is the discovery of bioactive lead compounds from the MediterraneanSea sponge Hemimycale Collumella (HC). HC sponge (189.0 g) was collected from Mdiq costs on the MediterraneanSea and extracted with methanol to yield (10 g) which was then subjected to fractionation. A bio-guided protocol wasapplied through evaluation of 2,2-diphenyl-1-picrylhydrazyl (DPPH) scavenging and Oxygen Radical Absorbance Capacity(ORAC), α-amylase, β-glucosidase, pancreatic lipase inhibition as well as anti-collagenase, anti-elastase, antityrosinaseand cytotoxic activity. 2,3-O-Hexahydroxydiphenoyl-(α/β)-glucose (1) and gentisic acid 2-O-β-glucoside (2) were isolatedfrom the water fraction, quercetin-3-O-β-glucopyranoside (3), kaempferol 3-O-β-glucopyranoside (4) and isorhamnetin3-O-β-glucopyranoside (5) from n-butanol fraction, gallic acid (6) from ethyl acetate fraction and gallic acid-3-methylether (7) from methylene chloride fraction. Compound 5 had the highest DPPH and ORAC activity. Compounds 1–5had promising lipase inhibition activities which exceeded that of the standard Orlistat, while compounds 1–7 showedanti-tyrosinase activity higher than that of the standard Hydroquinone monomethyl ether. This is the first report forevaluation of the biological activities of 2, 3-O-hexahydroxydiphenoyl-(α/β)-glucose (1), gentisic acid 2-O-β-glucoside(2) and gallic acid-3-methyl ether (7).