Rapid green synthesis of non‑cytotoxic silver nanoparticles using aqueous extracts of ’Golden Delicious’ apple pulp and cumin seeds with antibacterial and antioxidant activity

摘要

A simple, facile and rapid microwave irradiated system was applied to synthesize silver nanoparticles using ’Golden Delicious’apple pulp (Malus domestica) and cumin (Cuminum cyminum) seed extracts. The phytosynthesized AgNPs werecharacterized by Ultraviolet–Visible Spectroscopy (UV–vis), Fourier transform infrared (FTIR), X-ray Diffraction (XRD) TransmissionElectron Microscopy (TEM) and Zeta sizer analysis. In the study, the presence of face-centered cubic crystallinestructured metallic silver in AgNPs from apple and cumin extracts and the monodisperse nature of AgNPs with the sizedistribution range of 5.46–20 nm and 1.84–20.57 nm were confirmed, respectively. This study established an efficientgreen synthesis approach that created so far, the smallest silver nanoparticles by using these two extracts. According tothe results obtained, AgNPs synthesized using both extracts were non-toxic against L929 mouse fibroblast cells, whilethey were effective against both Gram-positive (Staphylococcus aureus) and Gram-negative (Escherichia coli) bacteria witha greater effect on S. aureus. Moreover, AgNPs synthesized through cumin extract exhibited a higher ABTS scavengingability (96.43 ± 0.78% at 160 μg/mL) in comparison to apple pulp extract mediated AgNPs, while both AgNPs showedlower activity for DPPH (27.84 ± 0.56% and 13.12 ± 0.32% from cumin seed and apple pulp extracts, respectively). Insummary, our results suggest the green non-cytotoxic AgNPs synthesized in this study could be a promising templatefor further biological and clinical applications.