β‑Cyclodextrin‑PANI decorated pencil graphite electrode for the electrochemical sensing of morin in almonds and mulberry leaves

摘要

Morin (3,2,4,5,7-pentahydroxyflavone) is one of the natural flavonoids which is present in a variety of fruits and herbs. β-cyclodextrin (β-CD) and polyaniline (PANI) decorated Pencil graphite electrode (PGE) has been successfully used as a sensitive and conducting electrode for the determination of morin. The hydroxyl groups of β-CD attract the analyte towards the modified electrode through hydrogen bonding. Cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS) techniques were employed to study the electrochemical properties of the modified electrodes. The enhanced surface roughness of β-CD-PANI/PGE has resulted in the increase of electrocatalytic activity of electrode towards the analyte. Opitical profilometric studies were performed to evaluate the surface roughness of electrodes and differential pulse votammetry (DPV) was used for the quantitative analysis of morin. Scanning electron microscopy (SEM), Raman spectroscopy and Fourier transform infrared (FTIR) spectroscopy were carried out to know the physicochemical characteristics of the modified electrodes. The experimental conditions such as scan rate, pH and concentration were optimized. The electrochemical process was found to be adsorption controlled and irreversible from scan rate studies. Under optimal conditions, the linear dynamic range for the quantification of morin was found to be 1.17–32 nM. The low detection limit (0.38 nM) indicates ultrasensitivity of the proposed method. The suggested method has been effectively employed for the determination of morin in almonds and mulberry leaves.