Bioprocess development for production of a process‑apt xylanase with multifaceted application potential for a range of industrial processes

摘要

Discovery of newovel enzymes with process-apt features has been a continuous practice. A wide range pH-stable andthermostable xylanase was reported hitherto from a newly isolated fungal strain Aspergillus terreus S9, from mushroomcompost. This is the first ever report where-in a pH-stable and thermostable xylanase is being reported from a strain ofA. terreus. Solid state fermentation was executed on agro-industrial residues for xylanase production. Maximum xylanaseproduction was supported by wheat bran (40 U/ml), however, substantial xylanase yield was observed on other residueslike corn cob powder (38.4 U/ml), cotton cake (38 U/ml), malt (37.6 U/ml) and almond hulls (35.2 U/ml). Almond hulls arebeing reported for the first time as the substrate for xylanase production. Plackett–Burman design was used to earmarkthe most effective process variables (wheat bran, medium pH, and incubation time), and the same were optimized bydesign of experiments (DoE) based on response surface methodology to achieve a yield enhancement of 1.82-fold. A.terreus S9 xylanase exhibited an excellent and brisk clarification of mosambi and orange juices. DoE based optimizationof process parameters (enzyme dose, treatment time and incubation temperature) resulted in 1.23-fold and 1.28-foldenhanced orange and mosambi juice clarification, respectively. Furthermore, admirable dough raising capacity and pulpbrightening ability of A. terreus S9 xylanase shows its promising potential for bread making, and eco-friendly biobleachingprocesses, respectively. The xylanase must be in-depth investigated to decipher the molecular mechanisms of diverseapplications.