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首页> 外文期刊>Scientific reports. >Endometrial Mesenchymal Stem/Stromal Cells Modulate the Macrophage Response to Implanted Polyamide/Gelatin Composite Mesh in Immunocompromised and Immunocompetent Mice
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Endometrial Mesenchymal Stem/Stromal Cells Modulate the Macrophage Response to Implanted Polyamide/Gelatin Composite Mesh in Immunocompromised and Immunocompetent Mice

机译:子宫内膜间充质干细胞/基质细胞调节免疫受损和免疫功能小鼠对植入的聚酰胺/明胶复合网的巨噬细胞反应。

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摘要

The immunomodulatory properties of human endometrial mesenchymal stem cells (eMSC) have not been well characterised. Initial studies showed that eMSC modulated the chronic inflammatory response to a non-degradable polyamide/gelatin mesh in a xenogeneic rat skin wound repair model, but the mechanism remains unclear. In this study, we investigated the immunomodulatory effect of eMSC on the macrophage response to polyamide/gelatin composite mesh in an abdominal subcutaneous wound repair model in C57BL6 immunocompetent and NSG (NOD-Scid-IL2Rgamma null ) immunocompromised mice to determine whether responses differed in the absence of an adaptive immune system and NK cells. mCherry lentivirus-labelled eMSC persisted longer in NSG mice, inducing longer term paracrine effects. Inclusion of eMSC in the mesh reduced inflammatory cytokine (Il-1β, Tnfα) secretion, and in C57BL6 mice reduced CCR7+ M1 macrophages surrounding the mesh on day 3 and increased M2 macrophage marker mRNA (Arg1, Mrc1, Il10) expression at days 3 and 7. In NSG mice, these effects were delayed and only observed at days 7 and 30 in comparison with controls implanted with mesh alone. These results show that the differences in the immune status in the two animals directly affect the survival of xenogeneic eMSC which leads to differences in the short-term and long-term macrophage responses to implanted meshes.
机译:人子宫内膜间充质干细胞(eMSC)的免疫调节特性尚未很好地表征。初步研究表明,eMSC在异种大鼠皮肤伤口修复模型中调节了对不可降解的聚酰胺/明胶网的慢性炎症反应,但机制尚不清楚。在这项研究中,我们研究了在具有C57BL6免疫能力和NSG(NOD-Scid-IL2Rgamma null)免疫力低下的小鼠的腹部皮下伤口修复模型中,eMSC对聚酰胺/明胶复合网对巨噬细胞应答的免疫调节作用,以确定在小鼠中,应答是否有所不同缺乏适应性免疫系统和NK细胞。 mCherry慢病毒标记的eMSC在NSG小鼠中持续更长的时间,从而引起长期的旁分泌作用。在网状细胞中包含eMSC减少了炎性细胞因子(Il-1β,Tnfα)的分泌,在C57BL6小鼠中,第3天减少了网状细胞周围的CCR7 + M1巨噬细胞,在第3天和第7天增加了M2巨噬细胞标志物mRNA(Arg1,Mrc1,Il10)的表达。 7.在NSG小鼠中,与仅植入网状细胞的对照组相比,这些作用被延迟,并且仅在第7天和第30天才观察到。这些结果表明,两只动物的免疫状态差异直接影响异种eMSC的存活,这导致了对植入网的短期和长期巨噬细胞应答的差异。

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