2',3'-Dialdehyde of ATP: a specific, irreversible inhibitor of component A3 of the methylreductase system of Methanobacterium thermoautotrophicum.

摘要

Among 17 purine and ATP derivatives tested, 3 were found to totally inhibit the methyl coenzyme M methylreductase system of Methanobacterium thermoautotrophicum at a concentration of 5 mM; these derivatives were 8-azido-ATP, alpha, beta-thio-ADP and 2',3'-dialdehyde-ATP. 2',3'-Dialdehyde-ATP specifically and irreversibly bound to component A3 of the methylreductase system during ATP activation of the system.