首页> 外文期刊>Journal of bacteriology >Development of a gene cloning system for Streptomyces hygroscopicus subsp. yingchengensis, a producer of three useful antifungal compounds, by elimination of three barriers to DNA transfer.
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Development of a gene cloning system for Streptomyces hygroscopicus subsp. yingchengensis, a producer of three useful antifungal compounds, by elimination of three barriers to DNA transfer.

机译:吸水链霉菌亚种基因克隆系统的开发。通过消除DNA转移的三个障碍,生产三种有用的抗真菌化合物的yingchengensis。

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摘要

Streptomyces hygroscopicus 10-22 could not be transformed with any of the commonly used Streptomyces plasmid vectors and was resistant to plaque formation by the Streptomyces phages phi C31 and R4. Repeated selection resulted in the isolation of derivatives of S. hygroscopicus 10-22 that could be transformed with pIJ101- and pJV1-derived cloning vectors and of restriction-deficient derivatives that could accept DNA propagated in Streptomyces lividans 66. These new strains, which include three that still produce the original antibiotics, can be used as hosts for gene cloning. Insertion of nonreplicating vectors by homologous recombination and transposition of Tn4560 were demonstrated in S. hygroscopicus 10-22.
机译:吸水链霉菌10-22不能用任何常用的链霉菌质粒载体转化,并且对链霉菌噬菌体phi C31和R4的噬菌斑形成具有抗性。重复选择导致分离出可用pIJ101和pJV1克隆载体转化的吸水链霉菌10-22衍生物,以及可以接受在青霉链霉菌66中繁殖的DNA的限制性缺陷衍生物。这些新菌株包括仍然可以产生原始抗生素的三种,可以用作基因克隆的宿主。在吸水链霉菌10-22中证明了通过同源重组插入非复制载体和Tn4560的转座。

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