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首页> 外文期刊>Journal of cell biology >Changes in the Activities of Aldolase and of D-Glyceraldehyde-3-Phosphate Dehydrogenase during the Mitotic Cycle in Microspores of Lilium longiflorum
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Changes in the Activities of Aldolase and of D-Glyceraldehyde-3-Phosphate Dehydrogenase during the Mitotic Cycle in Microspores of Lilium longiflorum

机译:长瓣百合小孢子有丝分裂周期中醛缩酶和D-甘油醛-3-磷酸脱氢酶活性的变化

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Microspores of Lilium longiflorum were isolated at various stages of development surrounding the mitotic interval and were analyzed for changes in the activities of D-glyceraldehyde-3-phosphate dehydrogenase and aldolase. Fructose 1,6 diphosphate was used as substrate. Activities were measured by the increase in optical density due to the reduction of diphosphopyridine nucleotide.It was found that mitosis occurs during the minimal activity of both aldolase and D-glyceraldehyde-3-phosphate, thus indicating that heightened glycolytic capacity is not necessarily related to mitosis. It was also found that soluble-SH levels were highest when the enzymes were least active. It appeared, therefore, that the "—SH enzymes" are not necessarily activated intracellularly by high concentrations of soluble thiol. These results are discussed in connection with the theory that soluble-SH compounds stimulate glycolysis and in this way initiate mitosis.
机译:在有丝分裂间隔的不同发育阶段,分离了长百合百合的小孢子,并分析了D-甘油醛-3-磷酸脱氢酶和醛缩酶活性的变化。使用果糖1,6二磷酸作为底物。活性是通过二磷酸吡啶核苷酸的减少导致的光密度的增加来测量的,发现有丝分裂发生在醛缩酶和D-甘油醛-3-磷酸的最小活性期间,因此表明糖酵解能力的提高不一定与有丝分裂。还发现当酶的活性最低时,可溶性SH水平最高。因此,似乎“ -SH酶”不一定被高浓度的可溶性硫醇在细胞内激活。结合可溶性SH化合物刺激糖酵解并以此方式引发有丝分裂的理论讨论了这些结果。

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